With newer versions of Huygens, the Gaussian blur prefilter is no longer needed. This outcome is ideal because it ensures that we and others will obtain excellent results with Huygens even under suboptimal imaging conditions. We find that this change completely solves the problems reported here for both real and simulated confocal data. After seeing the original version of the paper, scientists at SVI analyzed our image data and determined that deconvolution of very weak confocal signals could be improved by refining the background estimation procedure in Huygens. This revision has been prepared under somewhat unusual circumstances. Supplementary Figure 3 and Zip1_0.25%.tif and Zip1_100%.tif are the widefield image stacks used for Supplementary Figure 2 simulation_80x80x250_plus_noise.tif is the simulated confocal image stack used forįigure 3B simulation_40x40x120.tif is the simulated confocal image stack used for Video S2 Hmg1_1x.tif and Hmg1_8x.tif are the confocal image stacks used forįigure 2 simulation_80x80x250.tif is the simulated confocal image stack used for The following files are included: 4D_movie_1x.tif and 4D_movie_8x.tif are the 4D confocal data sets used for
The data referenced by this article are under copyright with the following copyright statement: Copyright: © 2017 Day KJ et al.ĭata associated with the article are available under the terms of the Creative Commons Zero "No rights reserved" data waiver (CC0 1.0 Public domain dedication).ĭataset 1: TIFF files for the experimental and simulated image data are provided in the compressed folder Original Image Files.zip.
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